Kulula kakhulu i-SYBR Green qPCR Master Mix
Kulula kakhulu i-SYBR Green qPCR Master Mix
Isingeniso
I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTPs,lyophilized) ilungele ukusetshenziswa, inhlanganisela eyinhloko ye-lyophilized yokukhulisa ukusebenza kahle kwe-PCR yesikhathi sangempela engasetshenziselwa ukutholwa ngokushesha kwamasampula e-DNA.Ingxube ihlanganisa isiqalo esishisayo esivinjwe kabili i-Super HP Taq DNA polymerase, PCR Buffer, MgCl2, SYBR Green dye nama-dNTP.Mane wenze kabusha ingxube eyinhloko ngokungeza amanzi ebanga le-PCR kanye nesifanekiso sakho kanye neziqalisi kuvolumu ephelele engu-20µl. Njengoba imikhiqizo ye-PCR ekhiqizwe i-lyophilized PCR Master Mix iqukethe ama-overhang angu-3'-A, ingaba ama-T-vectors ngokuqondile.Imikhiqizo ye-RT-qPCR ifanele ukulandelana.
Ukusebenza
Izinyathelo zokusebenza
| Inombolo yeCAT. | Isakhi | Ukucaciswa | Ubuningi | Qaphela |
| I-KY136-01 | I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) | 48T/ikhithi | 48 Amashubhu | I-strip yemithombo engu-8, 0.1mL |
| Amanzi e-PCR-grade | 1.5mL/Ithubhu | 1 Ithubhu | I-Cryotube, 2.0 mL | |
| I-KY136-02 | I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) | 48T/ikhithi | 48 Amashubhu | I-strip yemithombo engu-8, 0.2mL |
| Amanzi e-PCR-grade | 1.5mL/Ithubhu | 1 Ithubhu | I-Cryotube, 2.0 mL | |
| I-KY136-03 | I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) | 48T/ikhithi | 2 Amashubhu | I-Cryotube, 2.0 mL |
| Amanzi e-PCR-grade | 1.5mL/Ithubhu | 1 Ithubhu | I-Cryotube, 2.0 mL | |
| I-KY136-04 | I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) | 500T/ikhithi | 1Ithubhu | / |
| Amanzi e-PCR-grade | 10mL | 1Ibhodlela | / |
- Iphrofayili yomkhiqizo we-Hecin
