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I-PCR Master Mix elungele ukusetshenziswa
Isingeniso
I-Read-to-Use PCR Master Mix iyingxube elungele ukusetshenziswa, e-lyophilized master yokukhulisa ukusebenza kahle okuphezulu kwe-PCR yesikhathi sangempela engasetshenziselwa ukutholwa ngokushesha kwamasampula e-DNA. Le ngxube ihlanganisa i-hotstart evinjwe kabili i-Super HP Taq I-DNA polymerase, i-MgCl2 kanye ne-dNTPs.Vele wenze kabusha imiksi eyinhloko ngokwengeza amanzi ebanga le-PCR kanye nesifanekiso sakho, ama-Taqman probes kanye nama-primers kuvolumu isiyonke engu-20 µl.
Amapharamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ukulondolozwa okomile okuvaliwe, okungenayo umswakama. * Igama langaphambili lale khithi i-Realtime PCR Master Mix (ene-dNTPs, i-lyophilized).Inombolo yeCAT. Isakhi Ukucaciswa Ubuningi Qaphela I-KY133-01 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.1mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY133-02 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.2mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY133-03 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 2 Amashubhu I-Cryotube, 2.0 mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY133-04 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 500T/ikhithi 1Ithubhu / Amanzi e-PCR-grade 10mL 1Ibhodlela / Ukusebenza
•Ukusetshenziswa Okubanzi: Kuyasebenza ekutholweni okuningi.•Ukuzwela okuphezulu: Ukusebenza kahle kakhulu ekugxilweni kwezifanekiso eziphansi.•Okulula: Kuxutshwe ngaphambilini futhi kumahhala ukusebenzisa. -
I-Super-Easy SYBR Green RT-qPCR Master Mix
Isingeniso
I-SYBR Green RT-qPCR Master Mix elula kakhulu (ene-dNTPs, lyophilized) ilungele ukusetshenziswa, i-lyophilizedmastermix yokukhulisa ukusebenza kahle okuphezulu kwe-RT-qPCR engasetshenziswa ukuthola ngokushesha i-DNA noma amasampula e-RNA.I-Themixin ihlanganisa i-Super HP Taq DNA polymerase evinjwe kabili evinjwe kabili, i-M-MLV reverse transcriptase (RNaseH-), i-MgCl2, i-SYBR Udayi oluhlaza kanye nama-dNTP.Vele wenze kabusha imiksi eyinhloko ngokwengeza amanzi ebanga le-PCR ngokuhambisana nesifanekiso sakho ,i-Taqman iphenya kanye neziqalo kuvolumu isiyonke engu-20 µl.
Amapharamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ukulondolozwa okomile okuvaliwe, okungenayo umswakama. *Igama langaphambili lale kit i-Super-Easy SYBR Green RT-qPCR Master Mix (ene-dNTP, lyophilized). UkusebenzaInombolo yeCAT. Isakhi Ukucaciswa Ubuningi Qaphela I-KY135-01 I-SYBR Green RT-qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.1mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0 mL I-KY135-02 I-SYBR Green RT-qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.2mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0 mL I-KY135-03 I-SYBR Green RT-qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 2 Amashubhu I-Cryotube, 2.0 mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0 mL I-KY135-04 I-SYBR Green RT-qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 500T/ikhithi 1Ithubhu / Amanzi e-PCR-grade 10mL 1Ibhodlela / •Ukunemba: Ingozi ephansi yokutheleleka•Ukuzwela okuphezulu: Ukusebenza kahle kakhulu ekugxilweni kwezifanekiso eziphansi.•Okulula: Kuxutshwe ngaphambilini futhi kumahhala ukusebenzisa. -
Kulula kakhulu i-SYBR Green qPCR Master Mix
Isingeniso
I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTPs,lyophilized) ilungele ukusetshenziswa, inhlanganisela eyinhloko ye-lyophilized yokukhulisa ukusebenza kahle kwe-PCR yesikhathi sangempela engasetshenziselwa ukutholwa ngokushesha kwamasampula e-DNA.Ingxube ihlanganisa isiqalo esishisayo esivinjwe kabili i-Super HP Taq DNA polymerase, PCR Buffer, MgCl2, SYBR Green dye nama-dNTP.Mane wenze kabusha ingxube eyinhloko ngokungeza amanzi ebanga le-PCR kanye nesifanekiso sakho kanye neziqalisi kuvolumu ephelele engu-20µl. Njengoba imikhiqizo ye-PCR ekhiqizwe i-lyophilized PCR Master Mix iqukethe ama-overhang angu-3'-A, ingaba ama-T-vectors ngokuqondile.Imikhiqizo ye-RT-qPCR ifanele ukulandelana.
Amapharamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ukulondolozwa okomile okuvaliwe, okungenayo umswakama. *Igama langaphambili lale khithi lilula kakhulu i-SYBR Green qPCR Master Mix (ene-dNTPs, i-lyophilized)Inombolo yeCAT. Isakhi Ukucaciswa Ubuningi Qaphela I-KY136-01 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.1mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY136-02 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.2mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY136-03 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 48T/ikhithi 2 Amashubhu I-Cryotube, 2.0 mL Amanzi e-PCR-grade 1.5mL/Ithubhu 1 Ithubhu I-Cryotube, 2.0 mL I-KY136-04 I-SYBR Green qPCR Master Mix elula kakhulu (ene-dNTP, i-lyophilized) 500T/ikhithi 1Ithubhu / Amanzi e-PCR-grade 10mL 1Ibhodlela / Ukusebenza
•Ukusetshenziswa Okubanzi: Kuyasebenza ekutholweni okuningi.•Ukuzwela okuphezulu: Ukusebenza kahle kakhulu ekugxilweni kwezifanekiso eziphansi.•Okulula: Kuxutshwe ngaphambilini futhi kumahhala ukusebenzisa. -
Isinyathelo esisodwa se-RT-PCR Master Mix
Isingeniso
Isinyathelo esisodwa se-RT-PCR Master Mix ilungele ukusetshenziswa, i-lyophilized master mix yokukhulisa ukusebenza kahle okuphezulu kwe-RT-qPCR engasetshenziselwa ukutholwa ngokushesha kwamasampula e-DNA noma e-RNA.Ingxube ihlanganisa isiqalo esishisayo esivinjwe kabili i-Super HP Taq DNA polymerase, i-M-MLV reverse transcriptase (RNaseH-), MgCl2 anddNTPs.Vele wenze kabusha imiksi eyinhloko ngokwengeza amanzi ebanga le-PCR kanye nesifanekiso sakho ,u-Taqman uphenya futhi aqalise ingqikithi yevolumu engu-20 µl.
Amapharamitha
* Gcina ku -25 ℃ ~ 8 ℃.Ukulondolozwa okomile okuvaliwe, okungenayo umswakama. *Igama langaphambili lale khithi yi-One-step RT-qPCR Master Mix (ene-dNTPs, lyophilized).Inombolo yeCAT. Isakhi Ukucaciswa Ubuningi Qaphela I-KY132-01 I-One-Step RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.1mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0mL I-KY132-02 I-One-Step RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 48 Amashubhu I-strip yemithombo engu-8, 0.2mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0mL I-KY132-03 I-One-Step RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 48T/ikhithi 2 Amashubhu I-Cryotube, 2.0mL Amanzi e-PCR-grade 1.5mL/Tube 1Ithubhu I-Cryotube, 2.0mL I-KY132-04 I-One-Step RT-PCR Master Mix (ene-dNTPs, i-lyophilized) 500T/ikhithi 1Ithubhu / Amanzi e-PCR-grade 10mL/Tube 1Ithubhu / Ukusebenza
•Ukunemba: Ingozi ephansi yokutheleleka•Ukuzwela okuphezulu: Ukusebenza kahle kakhulu ekugxilweni kwezifanekiso eziphansi.•Okulula: Kuxutshwe ngaphambilini futhi kumahhala ukusebenzisa. -
Impahla eluhlaza
Ukuze kuhlangatshezwane nezidingo zemakethe ezibanzi, Sihlinzeka ngezinsizakalo zokukhiqiza izinto ezingavuthiwe zezinhlelo zokusabela ze-enzymatic ezinembayo ezithuthukisa ukunemba kwe-PCR.Siyajabula ukunethulela izinhlelo zethu zama-enzyme ayisithupha.
