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I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)

I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)

I-Ca16 Nucleic Acid Test Kit (PCR- fluorescence probe method) Isithombe Esifakiwe
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
  • I-Ca16 Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)

Incazelo emfushane:

Isingeniso

I-CA16 iyigciwane eliyinhloko elidala isifo se-Hand-mouth-foot (HFMD) ezinganeni.Ngokuvamile kuhambisana ne-Human Enterovirus 71 futhi kuvame kakhulu ezinganeni ezingaphansi kweminyaka engu-5 ubudala.Izimpawu zomtholampilo zokutheleleka kwe-CA16 yi-erythema, ama-papules namabhamuza amancane ezandleni nasezinyaweni zesiguli sengane, ehambisana nezilonda olimini kanye ne-oral mucosa.

Le khithi ihloselwe ukutholwa kokuthayipha kwekhwalithi ye-Coxsackievirus 16 nucleic acid ku-serum yomuntu noma amasampula e-plasma.Le khithi isebenzisa ufuzo olulondolozwe kakhulu lwe-5′UTR ohlotsheni lofuzo lwe-CA16 njengendawo eqondiwe, futhi idizayina ama-primers athile kanye nama-TaqMan fluorescent probes, futhi ibone ukutholakala okusheshayo nokuthayipha kwegciwane lodenga nge-PCR yesikhathi sangempela se-fluorescent.

Amapharamitha

Izingxenye 48T/Kit Izithako Eziyinhloko
Ingxube yokusabela ye-CA16/IC, i-lyophilized 2 amashubhu Iziqalo, ama-probe, i-PCR reaction buffer, dNTPs, Enzyme, njll.
Ukulawula okuhle kwe-CA16, i-lyophilized 1 ithubhu Izinhlayiya ze-pseudoviral ezihlanganisa ukulandelana okuqondiwe kanye nokulandelana kokulawula kwangaphakathi
Ukulawula okungalungile (Amanzi ahlanzekile) 3mL Amanzi ahlanzekile
Ukulawulwa kwangaphakathi kwe-RNA, i-lyophilized 1 ithubhu Izinhlayiya ze-pseudoviral ezihlanganisa i-MS2
* Uhlobo lwesampula: I-Serum noma i-Plasma.
* Amathuluzi okufaka isicelo: I-ABI 7500 Real-Time PCR System;I-Bio-Rad CFX96;I-Roche LightCycler480;Isistimu ye-PCR ye-SLAN.
* Isitoreji -25℃ kuya ku-8℃ esingavuliwe futhi sivikele ekukhanyeni izinyanga eziyi-18.

Ukusebenza

•Kushesha: Isikhathi esifushane kakhulu sokukhulisa i-PCR phakathi komkhiqizo ofanayo.
•Ukuzwela okuphezulu nokukhethekile: Kuthuthukisa ukuhlonzwa kusenesikhathi ukuze kutholakale ukwelashwa ngokushesha.
•Ikhono eliphelele lokulwa nokuphazamiseka.
•Ukutholwa kwezinhlobo eziningi ze-CA16: Uhlobo A/Uhlobo B(B1a,B2&B16)/Uhlobo C.

Izinyathelo zokusebenza


Imininingwane Yomkhiqizo

Amapharamitha

Landa

Omaka bomkhiqizo

Ikhithi Yokuhlola I-Ca16 Nucleic Acid(Indlela ye-PCR-fluorescence probe)

Isingeniso

I-CA16 iyigciwane eliyinhloko elidala isifo se-Hand-mouth-foot (HFMD) ezinganeni.Ngokuvamile kuhambisana ne-Human Enterovirus 71 futhi kuvame kakhulu ezinganeni ezingaphansi kweminyaka engu-5 ubudala.Izimpawu zomtholampilo zokutheleleka kwe-CA16 yi-erythema, ama-papules namabhamuza amancane ezandleni nasezinyaweni zesiguli sengane, ehambisana nezilonda olimini kanye ne-oral mucosa.

Le khithi ihloselwe ukutholwa kokuthayipha kwekhwalithi ye-Coxsackievirus 16 nucleic acid ku-serum yomuntu noma amasampula e-plasma.Le khithi isebenzisa ufuzo olulondolozwe kakhulu lwe-5′UTR ohlotsheni lofuzo lwe-CA16 njengendawo eqondiwe, futhi idizayina ama-primers athile kanye nama-TaqMan fluorescent probes, futhi ibone ukutholakala okusheshayo nokuthayipha kwegciwane lodenga nge-PCR yesikhathi sangempela se-fluorescent.

Ukusebenza

•Kushesha: Isikhathi esifushane kakhulu sokukhulisa i-PCR phakathi komkhiqizo ofanayo.
•Ukuzwela okuphezulu nokukhethekile: Kuthuthukisa ukuhlonzwa kusenesikhathi ukuze kutholakale ukwelashwa ngokushesha.
•Ikhono eliphelele lokulwa nokuphazamiseka.
•Ukutholwa kwezinhlobo eziningi ze-CA16: Uhlobo A/Uhlobo B(B1a,B2&B16)/Uhlobo C.
Izinyathelo zokusebenza

  • Okwedlule:
  • Olandelayo:
    • Izingxenye 48T/Kit Izithako Eziyinhloko
    Ingxube yokusabela ye-CA16/IC, i-lyophilized 2 amashubhu Iziqalo, ama-probe, i-PCR reaction buffer, dNTPs, Enzyme, njll.
    Ukulawula okuhle kwe-CA16, i-lyophilized 1 ithubhu Izinhlayiya ze-pseudoviral ezihlanganisa ukulandelana okuqondiwe kanye nokulandelana kokulawula kwangaphakathi
    Ukulawula okungalungile (Amanzi ahlanzekile) 3mL Amanzi ahlanzekile
    Ukulawulwa kwangaphakathi kwe-RNA, i-lyophilized 1 ithubhu Izinhlayiya ze-pseudoviral ezihlanganisa i-MS2
    * Uhlobo lwesampula: I-Serum noma i-Plasma.
    * Amathuluzi okufaka isicelo: I-ABI 7500 Real-Time PCR System;I-Bio-Rad CFX96;I-Roche LightCycler480;Isistimu ye-PCR ye-SLAN.
    * Isitoreji -25℃ kuya ku-8℃ esingavuliwe futhi sivikele ekukhanyeni izinyanga eziyi-18.
    • Iphrofayili yomkhiqizo we-Hecin
      LANDA
    Bhala umyalezo wakho lapha futhi usithumelele wona

    Umkhiqizoizigaba

    Chofoza u-enter ukuze useshe noma u-ESC ukuze uvale