I-EV Nucleic Acid Test Kit (indlela ye-PCR-fluorescence probe)
Incazelo emfushane:
Isingeniso
Izimpawu eziyinhloko zomtholampilo zokutheleleka kwe-EV yilezi: iziguli ezithelelekile, ikakhulukazi izingane, isikhumba kanye nolwelwesi lwamafinyila kanye nezilonda ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye, futhi eziningi zazo zihambisana nezimpawu zesistimu ezifana nomkhuhlane, i-anorexia, ukukhathala, kanye ukungabi naluzwelo.Ukutheleleka okuncane kungabangela isifo sohudo, umkhuhlane, ukuqubuka kwe-herpetic, i-aseptic meningitis, i-encephalitis, i-myocarditis, kanye nezimo ezinzima zingabonakala njenge-acute flaccid paralysis (AFP), i-pulmonary edema noma ukopha, ngisho nokufa.I-EV ihlasela kakhulu izinsana nezingane ezincane, ikakhulukazi izingane ezingaphansi kweminyaka engu-5 ubudala.
Le kit ihloselwe ukutholwa kokuthayipha kwekhwalithi ye-enterovirus nucleic acid ku-serum yomuntu noma amasampula e-plasma.Le khithi isebenzisa ufuzo olulondolozwe kakhulu lwe-5′UTR kufuzo lwe-EV njengendawo eqondiwe, futhi idizayina ama-primers athile kanye nama-TaqMan fluorescent probe, futhi ibone ukutholakala okusheshayo nokuthayipha kwegciwane lodenga nge-PCR yesikhathi sangempela se-fluorescent.
Amapharamitha
| Izingxenye | 48T/Kit | Izithako Eziyinhloko |
| Ingxube yokusabela ye-EV/IC, i-lyophilized | 2 amashubhu | Iziqalo, ama-probe, i-PCR reaction buffer, dNTPs, Enzyme, njll. |
| Ukulawula okuhle kwe-EV, i-lyophilized | 1 ithubhu | Izinhlayiya ze-pseudoviral ezihlanganisa ukulandelana okuqondiwe kanye nokulandelana kokulawula kwangaphakathi |
| Ukulawula okungalungile (Amanzi ahlanzekile) | 3mL | Amanzi ahlanzekile |
| Ukulawulwa kwangaphakathi kwe-RNA, i-lyophilized | 1 ithubhu | Izinhlayiya ze-pseudoviral ezihlanganisa i-MS2 |
| IFU | 1 iyunithi | Imanuwali Yeziqondiso Zomsebenzisi |
Ukusebenza
Izinyathelo zokusebenza
Ikhithi Yokuhlola I-EV Nucleic Acid(Indlela ye-PCR-fluorescence probe)
Isingeniso
Izimpawu eziyinhloko zomtholampilo zokutheleleka kwe-EV yilezi: iziguli ezithelelekile, ikakhulukazi izingane, isikhumba kanye nolwelwesi lwamafinyila kanye nezilonda ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye, futhi eziningi zazo zihambisana nezimpawu zesistimu ezifana nomkhuhlane, i-anorexia, ukukhathala, kanye ukungabi naluzwelo.Ukutheleleka okuncane kungabangela isifo sohudo, umkhuhlane, ukuqubuka kwe-herpetic, i-aseptic meningitis, i-encephalitis, i-myocarditis, kanye nezimo ezinzima zingabonakala njenge-acute flaccid paralysis (AFP), i-pulmonary edema noma ukopha, ngisho nokufa.I-EV ihlasela kakhulu izinsana nezingane ezincane, ikakhulukazi izingane ezingaphansi kweminyaka engu-5 ubudala.
Le kit ihloselwe ukutholwa kokuthayipha kwekhwalithi ye-enterovirus nucleic acid ku-serum yomuntu noma amasampula e-plasma.Le khithi isebenzisa ufuzo olulondolozwe kakhulu lwe-5′UTR kufuzo lwe-EV njengendawo eqondiwe, futhi idizayina ama-primers athile kanye nama-TaqMan fluorescent probe, futhi ibone ukutholakala okusheshayo nokuthayipha kwegciwane lodenga nge-PCR yesikhathi sangempela se-fluorescent.
Ukusebenza
Izinyathelo zokusebenza
| Izingxenye | 48T/Kit | Izithako Eziyinhloko |
| Ingxube yokusabela ye-EV/IC, i-lyophilized | 2 amashubhu | Iziqalo, ama-probe, i-PCR reaction buffer, dNTPs, Enzyme, njll. |
| Ukulawula okuhle kwe-EV, i-lyophilized | 1 ithubhu | Izinhlayiya ze-pseudoviral ezihlanganisa ukulandelana okuqondiwe kanye nokulandelana kokulawula kwangaphakathi |
| Ukulawula okungalungile (Amanzi ahlanzekile) | 3mL | Amanzi ahlanzekile |
| Ukulawulwa kwangaphakathi kwe-RNA, i-lyophilized | 1 ithubhu | Izinhlayiya ze-pseudoviral ezihlanganisa i-MS2 |
| IFU | 1 iyunithi | Imanuwali Yeziqondiso Zomsebenzisi |
- Iphrofayili yomkhiqizo we-Hecin
